Efficacy Of Annona Squamosa Extract Loaded on Silver Nanoparticles Against Echinococcus Granulosus

Abstract

The current study was aimed to determine the Efficacy of Annona squamosa extract loaded with silver nanoparticles against the protoscolex of Echinococcus granulosus in vitro. The study samples were divided into four groups, The experimental groups consisted of the control group, the group treated with the unbound plant extract, the group treated with the unbound silver nanoparticles, and the group treated with the plant extract bound to the silver nanoparticles. The result showed that at zero hour, the percentage of protoscolex vitality was (97 ,92 ,90) at the concentrations (0.2 ,0.4,0.6 mg/ml) for Annona squamata extract, free silver nanoparticles and silver nanoparticles loaded with Annona squamata extract respectively. The silver nanoparticles when loaded with Annona squamataextract was the most effective in reducing the viability of protoscoles which reach (0) % after (120) hours in concentration 0.6 mg/ml compared to the rest of the concentrations used in the study and control group, followed by free silver treatment group which reach (8.78) after 120 hours in concentration 0.4 mg/ml compare with (18.9%) for treatment with plant extract in the fifth day. The study concluded that all treatments were affected and had significant differences in reducing the vitality percentage under 0< 0.05. but the silver nanoparticles when loaded with Annona squamata extract was more sufficient in reducing the vitality of Echinococcus granulosus protoscolex in vitro.