Reduction of Heat Stress Effect on Hematological and Antioxidant Indicators by Acetaminophen and Vitamin C Supplement for Dairy Buffalo of Al-Chybaish Area

Abstract

This study was conducted in a field in Al-Chibayish Swamp, Thi-Qar Province, Iraq, from July 23, 2022 to August 21, 2022. The objective was to investigate the impact combination of acetaminophen and vitamin C adding in a water drink for several days a week to minimize the effects of summer heat stress (HS) and to examine its effects on hematologic biochemical and hormonal biomarkers relate to oxidative status of the body. Sixteen water buffalo, aged 4 to 7 years and weighing between 450 and 500 kg, were fed according to their dietary requirements and randomly assigned to four treatments (T1, T2, T3 and T4). , each treatment consisted of four animals. The first treatment (T1) drank water regularly (control group), while treatment T2, T3 and T4 drank water mixed with acetaminophen and vitamin C (AVC) at a dose of 500g/1000 liters for treatment 3, 5 and 7 consecutive days per week, respectively, over a 30-day period. The environmental climatic conditions (temperature, humidity and temperature-humidity index) were recorded daily at 15:00. Complete blood counts and several biochemical parameters (ALT, AST, ALP, urea and creatinine) were monitored, as well as cortisol and glutathione measurements as antioxidant markers. The results of the present study revealed that the administration of AVC significantly impacted the hematological indices and the total and differential leukocyte values. The 7-day AVC supplementation led to increased values of RBC, Hb, and HCT, while WBC, neutrophils, and monocytes values decreased, in comparison to the control group. A significant decrease (P≤0.05) in liver enzymes was appear in the fourth group showed lower levels of ALT, AST, and ALP compared to the other three groups after 10, 20, and 30 days of AVC administration, while the control group showed the highest values of these HS markers. Whereas, the results indicated there were no significant differences in renal function markers (creatinine and urea) along the study periods. While, treatments 2, 3, and 4 showed significant differences compared to the control group in cortisol hormone and glutathione enzyme levels. Animals treated with 7 days of AVC had the highest values, while the control group had the lowest values. According to above results can conclude that use of acetaminophen and vitamin c combinations is safe for buffaloes and reduce the deleterious effect of heat stress and improve water dairy buffalo physiology and oxidative status.