Effect of Water and Lipid Soluble Antioxidant on Oxidative Stress Biomarker and Lipid Profile in High Fat-Fed Rats

Abstract

Hyperlipidemia refers to elevated levels of lipids (such as cholesterol and triglycerides) in the blood. It is a common condition that can increase the risk of cardiovascular diseases, such as heart attacks and strokes. Managing hyperlipidemia typically involves lifestyle modifications and, in some cases, medication. The prevalence of obesity and type 2 diabetes mellitus (T2DM), contributing to the burden of Hyperlipidemia, is not projected to decline in the foreseeable future. A high-fat diet is associated with increased oxidative stress and free-radical activity. Oxidative stress plays a role in Hyperlipidemia development, and many antioxidants reverse the free radical action and delay liver damage, like vitamin C and alpha-lipoic acid, ubiquinone 10, and L-carnitine, 32 male and female rats weighing 150-200 grams were divided into four groups. The various antioxidants used in different combinations in treated group 3 were water-soluble vitamin C, L-carnitine, lipid-soluble antioxidants ubiquinone and alpha lipoic acid given to group 4 orally. The result of this study was a significant increase in Malondialdehyde MDA level in the high fat-fed group and a decrease in Superoxide dismutase SOD and Glutathione peroxidase GPx an elevation in a lipid profile, LDL, vLDL Triglyceride, and reduction in HDL, a significant reduction in Malondialdehyde level and increase in Superoxide dismutase and Glutathione peroxidase level and decrease Lipid profile level in antioxidant group 3 and 4. The role of antioxidants as protective agents against oxidative stress decreases free radicals’ activity by increasing the scavenging properties of antioxidants.