Line-1 Elements Gene Expression and Some Physiological Parameters Association with Diabetes Mellitus

Abstract

Line-1 element has been implicated in disease progression and carcinogenesis. It is uncertain how this element's dysregulation contributes to the onset of diabetes, however, it can involve genomic instability and abnormal gene regulation. This project sought to examine transcript expression of line-1 in blood cells of diabetes mellitus patients and whether the line-1 associated with metabolic markers. The results revealed that blood glucose level, HbA1c were increased significantly in diabetes patients than healthy (p≤0.001). Additionally, TC, TG, LDL and VLDL concentration were significantly evaluated in patients compare to control (p≤0.05, p≤0.001, respectively). However, HDL serum level was decreased in diabetic patients (p≥0.05). In the case line-1, the relative fold change (2 -∆∆CT), the finding revealed that line-1 expression was significantly increased by (p≤0.001) fold in diabetes mellitus. In conclusion, the line-1 was up regulated in the diabetes patients and it was accompanied by change of physiological parameters levels. The there was a positive correlation line-1 transcription with glucose and HbA1c concentrations, in addition to lipid profile including TC, TG and LDL showed a positive relationship with line-1 transcription. In contrast, there were negative correlation line-1 expression with HDL and VLDL. There were positive relationship between mRNA line-1 expression and HDL/LDL as well as LDL/HDL ratio.