Evaluation the Serum Level of the Carbamylated Protein Antibody Compare with the Anti-Citrullinated Peptide Antibody and Rheumatoid Factor Antibody in Rheumatoid Arthritis Patients

Authors

  • Zahraa Qasim Ali (MSc) Medical Microbiology and Immunology, Karbala University\Collage of Medicine
  • Dr. Alaa Saad Alattabi FICMS/ Pathology and Immunology, Karbala University\Collage of Medicine https://orcid.org/0000-0001-9029-8936
  • Dr. Dhamiaa Maki Hamza Medical Microbiology, Karbala University\Collage of Medicine
  • Dr. Murtadha Abdullah Abbood AL-gaber Medical Microbiology and Immunology, Karbala University\Collage of Medicine https://orcid.org/0000-0002-2923-3604
  • Dr. Firas A. Zenki (MSc Rheumatology) Karbala Health Directory

Keywords:

RA, RF, ACPA, AntiCarP, DAS28-CRP

Abstract

Background: Rheumatoid arthritis is an autoimmune disease that causes irreversible joint destruction due to persistent synovitis. ACPA and RF are gold standard antibodies for diagnosis, many studies shown that Anti-CarP can be utilized with ACPA.

Objective: Comparison of Anti-CarP with ACPA and RF serum levels in RA patients based on disease severity.

Materials and Methods: Case-control study includes 270 subjects from October 2022 to April 2023 in Al-Mujtaba Hospital/Rheumatology Unit, separated into 180 RA patients and 90 controls, then into 90 seropositive and 90 seronegative RA cases. All patients consented to ACR 2010 rheumatologists' diagnoses; venous blood samples were collected and split into sodium citrate tubes for Westergren ESR and gel tubes for serological assays, C-RP and RF employing Nephelometry, ACPA, and anti-CarP by ELISA. SPSS 26 was used for statistical analysis.

Results: RA cases were 180, divided into 90 seropositive and 90 seronegative RA patients, mean of Anti-CarP highly significant in seronegative than seropositive at P.value (0.000), in mild and moderate DAS28-CRP at P.value (0.001), and significant in regulars treatment than irregulars at P.value (0.047), while ACPA and RF highly significant in seropositive than seronegative at P.value (0.000), in severe DAS28-CRP at P.value less than (0.01), and in irregulars treatment than regulars at P.value (0.001).

Conclusions: Anti-CarP Ab associated with RA patients especially seronegative RA so may be useful as diagnostic marker complementary with RF and ACPA, associated with mild and moderate DAS28-CRP and regulars treatment compare to the ACPA and RF so could be used as prognostic marker for disease severity.

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Published

2023-09-21

How to Cite

Zahraa Qasim Ali, Dr. Alaa Saad Alattabi, Dr. Dhamiaa Maki Hamza, Dr. Murtadha Abdullah Abbood AL-gaber, & Dr. Firas A. Zenki. (2023). Evaluation the Serum Level of the Carbamylated Protein Antibody Compare with the Anti-Citrullinated Peptide Antibody and Rheumatoid Factor Antibody in Rheumatoid Arthritis Patients. World of Science: Journal on Modern Research Methodologies, 2(9), 32–40. Retrieved from https://univerpubl.com/index.php/woscience/article/view/2526