Evaluation the Serum Level of the Carbamylated Protein Antibody Compare with the Anti-Citrullinated Peptide Antibody and Rheumatoid Factor Antibody in Rheumatoid Arthritis Patients
Keywords:
RA, RF, ACPA, AntiCarP, DAS28-CRPAbstract
Background: Rheumatoid arthritis is an autoimmune disease that causes irreversible joint destruction due to persistent synovitis. ACPA and RF are gold standard antibodies for diagnosis, many studies shown that Anti-CarP can be utilized with ACPA.
Objective: Comparison of Anti-CarP with ACPA and RF serum levels in RA patients based on disease severity.
Materials and Methods: Case-control study includes 270 subjects from October 2022 to April 2023 in Al-Mujtaba Hospital/Rheumatology Unit, separated into 180 RA patients and 90 controls, then into 90 seropositive and 90 seronegative RA cases. All patients consented to ACR 2010 rheumatologists' diagnoses; venous blood samples were collected and split into sodium citrate tubes for Westergren ESR and gel tubes for serological assays, C-RP and RF employing Nephelometry, ACPA, and anti-CarP by ELISA. SPSS 26 was used for statistical analysis.
Results: RA cases were 180, divided into 90 seropositive and 90 seronegative RA patients, mean of Anti-CarP highly significant in seronegative than seropositive at P.value (0.000), in mild and moderate DAS28-CRP at P.value (0.001), and significant in regulars treatment than irregulars at P.value (0.047), while ACPA and RF highly significant in seropositive than seronegative at P.value (0.000), in severe DAS28-CRP at P.value less than (0.01), and in irregulars treatment than regulars at P.value (0.001).
Conclusions: Anti-CarP Ab associated with RA patients especially seronegative RA so may be useful as diagnostic marker complementary with RF and ACPA, associated with mild and moderate DAS28-CRP and regulars treatment compare to the ACPA and RF so could be used as prognostic marker for disease severity.