Detection of the Capsule Production, Biofilm formation in Escherichia coli isolated from different sources in Kirkuk city

Abstract

This study was carriedf out in Kirkuk city from November 2021 to April 2022. The study included,v155 specimens of patients (50) urine specimens , (55 ) sputum specimens,( 25) wound specimens and (25) burn specimens ,who attended Azadi Teaching Hospital in Kirkuk city. This study came to identify some virulence factors for Escherichia coli, four kinds of specimens were collected from patients, Smear samples were placed in sterile collection tubes containing transport media, and then transported to the laboratory for culture on different selective media for identification. the Bacterial isolates were diagnosed using traditional methods, the results showed that (37) bacterial isolates were biofilm-producing, by various grades, so (13) isolates formed the biofilm with a strong adherent grades,. , (18) isolates forming a biofilm with a moderately adherent, and (6) isolates forming a biofilm with a weakly adherent grades, ,as the results showed that (29) bacterial isolates had the capsule. The Antimicrobial Agents test results showed that 42 (100%) isolates were resistants to Amoxicillin, 41 (98%) isolates were resistants to Cefotaxime, 35 (83%) isolates were resistants to Cefexime, and 34 (81%) were resistants to the Cefepime,33 (78%) isolates were resistants to the Ceftriaxone,, (27) (64%) isolates were resistants to the Gentamicin, and 26 (62%) isolates were resistants to the Doxycycline (24) (57%), isolate was resistants’ to the Amikacin, And 10 (24%) isolates were resistants to Imipenem ,and 16 (38%) isolates were resistants to Levofloxacin