Detection of Chemical Compounds (Secondary Metabolites) of Alcoholic Extract of Cladosporium Cucumerinum using Gas Chromatogram-Mass Spectrometry GC-MS

Abstract

The current study aimed to detect the chemical content of the active metabolites of the alcoholic extract (ethyl ester) of Cladosporium cucumerinum (endophytic fungi) isolated from the leaves of the olive plant Olea Europa, where gas chromatographic analysis of the alcoholic extract of Cladosporium cucumerinum showed many chemical compounds, including 7 The largest percentage was the compound undecenoic acid ethyl ester 10- which recorded the highest percentage as it was 40.28%, followed by the compound Choroundecanoic acid, chloromethyl at a rate of 39. 38%, where these two compounds constitute the majority of the chemical content of the alcoholic extract of the mushroom Cladosporium cucumerinum, followed by the compound Ricinoleic acid Which recorded a ratio of 11.77%, then the compound Heptadecanoic acid, ethyl ester, its percentage was 2.24, the fifth compound that was diagnosed was Behenic acid (methyl,19-methyl-eicosanoate) and its rate was 2.71%, and Methyl,12-hydroxy-9-octadecenoate At a rate of 2.22%, and Nitromethane at a rate of 1.37%.